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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
11/06/2020 |
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Actualizado : |
22/02/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DO CARMO, M.; CARDOZO, G.; MECATTI, F.; SOCA, P.; HIRATA, M. |
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Afiliación : |
MARTÍN DO CARMO, Departamento de Sistemas Agrarios y Paisajes Culturales, Centro Universitario de la Región Este, Rocha, Uruguay.; GERONIMO AGUSTIN CARDOZO CABANELAS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FULVIA MECATTI, Department of Statistics, Università degli Studi di Milano-Bicocca.; PABLO SOCA, Universidad de la República, School of Agronomy, EEMAC.; MASAHIKO HIRATA, Department of Animal and Grassland Sciences, University of Miyazaki. |
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Título : |
Number of samples for accurate visual estimation of mean herbage mass in Campos grasslands. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Agronomy Journal, July 2020, Vol. 112(4), p. 2734-2740. Doi: https://doi.org/10.1002/agj2.20237 |
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DOI : |
DOI: 10.1002/agj2.20237 |
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Idioma : |
Inglés |
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Notas : |
Article history: Manuscript received: 23 October 2019. Manuscript revised: 23 March 2020. Manuscript accepted: 01 April 2020. Accepted manuscript online: 09 April 2020. Version of Record online: 19 May 2020. Published July 2020. |
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Contenido : |
The number of samples is a major issue when estimating the mean herbage mass of grazed paddocks. The aim of this study was to assess the number of samples required for accurate visual estimation of mean herbage mass in relation to the herbage mass heterogeneity and size of paddocks. Data were collected across scales of space and time (273 sampling events) from paddocks on Campos grasslands in Uruguay, using the visual estimation technique. The mean herbage mass of the paddocks ranged from 270 to 6350 kg DM ha−1 with coefficient of variation (CV) of 0.13 to 1.26. Twenty-four events representing four levels of herbage mass heterogeneity (CV = 0.3, 0.5, 0.7 and 1.0) × three levels of paddock size (small, 5?13 ha; medium, 41?67 ha; large, 100?140 ha) were chosen (two replicates per group), and analyzed for the probability that the estimation error exceeded 10% of the mean (10% error probability) using the bootstrap technique. The number of samples required for
controlling the 10% error probability below 0.1 increased gradually from 50 to 150 per paddock as the CV increased from 0.3 to 0.7, then sharply to 350 until the CV increased to 1.0, with no effect of paddock size. Taking account of the distribution of CV (< 0.7 in nearly 80% of the events), we propose a general recommendation to take a minimum of 150 samples per paddock for accurate estimation of mean herbage mass in Campos grasslands irrespective of the size of paddocks. |
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Palabras claves : |
ANIMAL PRODUCTION; E-TANIN; ESTIMACION DE BIOMASA; FIELD IMPROVEMENT; LOTUS PEDUNCULATUS; MEJORAMIENTO DE CAMPO; MEJORAMIENTO DE CAMPO NATURAL; PRODUCCIÓN ANIMAL. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02569naa a2200289 a 4500
001 1061106
005 2021-02-22
008 2020 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1002/agj2.20237$2DOI
100 1 $aDO CARMO, M.
245 $aNumber of samples for accurate visual estimation of mean herbage mass in Campos grasslands.$h[electronic resource]
260 $c2020
500 $aArticle history: Manuscript received: 23 October 2019. Manuscript revised: 23 March 2020. Manuscript accepted: 01 April 2020. Accepted manuscript online: 09 April 2020. Version of Record online: 19 May 2020. Published July 2020.
520 $aThe number of samples is a major issue when estimating the mean herbage mass of grazed paddocks. The aim of this study was to assess the number of samples required for accurate visual estimation of mean herbage mass in relation to the herbage mass heterogeneity and size of paddocks. Data were collected across scales of space and time (273 sampling events) from paddocks on Campos grasslands in Uruguay, using the visual estimation technique. The mean herbage mass of the paddocks ranged from 270 to 6350 kg DM ha−1 with coefficient of variation (CV) of 0.13 to 1.26. Twenty-four events representing four levels of herbage mass heterogeneity (CV = 0.3, 0.5, 0.7 and 1.0) × three levels of paddock size (small, 5?13 ha; medium, 41?67 ha; large, 100?140 ha) were chosen (two replicates per group), and analyzed for the probability that the estimation error exceeded 10% of the mean (10% error probability) using the bootstrap technique. The number of samples required for controlling the 10% error probability below 0.1 increased gradually from 50 to 150 per paddock as the CV increased from 0.3 to 0.7, then sharply to 350 until the CV increased to 1.0, with no effect of paddock size. Taking account of the distribution of CV (< 0.7 in nearly 80% of the events), we propose a general recommendation to take a minimum of 150 samples per paddock for accurate estimation of mean herbage mass in Campos grasslands irrespective of the size of paddocks.
653 $aANIMAL PRODUCTION
653 $aE-TANIN
653 $aESTIMACION DE BIOMASA
653 $aFIELD IMPROVEMENT
653 $aLOTUS PEDUNCULATUS
653 $aMEJORAMIENTO DE CAMPO
653 $aMEJORAMIENTO DE CAMPO NATURAL
653 $aPRODUCCIÓN ANIMAL
700 1 $aCARDOZO, G.
700 1 $aMECATTI, F.
700 1 $aSOCA, P.
700 1 $aHIRATA, M.
773 $tAgronomy Journal, July 2020, Vol. 112(4), p. 2734-2740. Doi: https://doi.org/10.1002/agj2.20237
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
24/01/2020 |
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Actualizado : |
10/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MACÍAS-RIOSECO, M.; CAFFARENA, D.; FRAGA, M.; SILVEIRA, C.S.; GIANNITTI, F.; CANTÓN, G.; HECKER, Y.P.; SUANES, A.; RIET-CORREA, F. |
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Afiliación : |
MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República, Facultad de Veterinaria, Montevideo, Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; University of Minnesota, Veterinary Population Medicine Department, MN, United States; GERMÁN CANTÓN, Instituto Nacional de Tecnología Agropecuaria, Balcarce, Argentina; YANINA P. HECKER, Consejo Nacional de Investigaciones Científicas y Técnicas, Balcarce, Argentina; ALEJANDRA SUANES, Ministerio de Ganadería Agricultura y Pesca, Montevideo. Dirección de Laboratorios Veterinarios, Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd. (Technical note). [Brote de abortos causado por Campylobacter fetus subespecie venerealis y Neospora caninum en un hato bovino lechero. (Nota de investigación)]. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Revista Mexicana De Ciencias Pecuarias, 2019, Volume 10, Issue 4, 2019, Pages 1054-1063. OPEN ACCESS. Doi: 10.22319/rmcp.v10i4.5008 |
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ISSN : |
2007-1124 |
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DOI : |
10.22319/rmcp.v10i4.5008 |
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Idioma : |
Inglés |
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Notas : |
Article history: Recibido: 02 Agosto 2018. / Aceptado: 28 Agosto 2018. |
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Contenido : |
ABSTRACT.
In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome.
© 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved.
RESUMEN.
En noviembre de 2015 ocurrió un brote de abortos en un hato lechero comercial de 650 vacas Holstein en el departamento de Florida, Uruguay. Cuarenta y cinco (45) vacas abortaron en un lapso de 3 semanas. Cinco fetos fueron sometidos a un examen patológico macro y microscópico y a pruebas microbiológicas. Un feto tenía epicarditis fibrinosa y peritonitis, así como bronconeumonía neutrofílica. Se detectó Campylobacter fetus subesp. venerealis utilizando inmunofluorescencia directa; se lo aisló e identificó mediante PCR y secuenciación del 16S rDNA en el líquido abomasal y en el pulmón. El examen histológico de otros dos fetos reveló encefalitis necrotizante no supurativa, miositis linfohistiocítica y miocarditis, y nefritis linfocítica intersticial. En estos fetos se detectó intralesionalmente el antígeno de N. caninum mediante análisis inmunohistoquímico, y se amplificó el ADN de N. caninum mediante PCR en tejido cerebral fijado con formalina y embebido en parafina. Se detectaron anticuerpos contra N. caninum mediante inmunofluorescencia indirecta en 10 de 27 vacas, con títulos de entre 1/200 y 1/3200. Los resultados indican que dos microorganismos abortígenos pueden coexistir y provocar abortos contemporáneos en un hato. Subrayamos la importancia de realizar pruebas diagnósticas múltiples en diversas madres abortadas y fetos del mismo hato para obtener una confirmación etiológica del síndrome de aborto bovino. MenosABSTRACT.
In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome.
© 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved.
RESUMEN.
En noviembre de 2015 ocurrió un brote de abortos en un hato lechero... Presentar Todo |
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Palabras claves : |
Bovine abortion; Campylobacter fetus subsp. venerealis; Diagnosis of abortion; Neospora caninum; PLATAFORMA SALUD ANIMAL. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14066/1/5008-14394-1-PB.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/14067/1/5008-14395-1-PB.pdf
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Marc : |
LEADER 04147naa a2200313 a 4500
001 1060687
005 2020-02-10
008 2019 bl uuuu u00u1 u #d
022 $a2007-1124
024 7 $a10.22319/rmcp.v10i4.5008$2DOI
100 1 $aMACÍAS-RIOSECO, M.
245 $aAbortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd. (Technical note). [Brote de abortos causado por Campylobacter fetus subespecie venerealis y Neospora caninum en un hato bovino lechero. (Nota de investigación)].$h[electronic resource]
260 $c2019
500 $aArticle history: Recibido: 02 Agosto 2018. / Aceptado: 28 Agosto 2018.
520 $aABSTRACT. In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome. © 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved. RESUMEN. En noviembre de 2015 ocurrió un brote de abortos en un hato lechero comercial de 650 vacas Holstein en el departamento de Florida, Uruguay. Cuarenta y cinco (45) vacas abortaron en un lapso de 3 semanas. Cinco fetos fueron sometidos a un examen patológico macro y microscópico y a pruebas microbiológicas. Un feto tenía epicarditis fibrinosa y peritonitis, así como bronconeumonía neutrofílica. Se detectó Campylobacter fetus subesp. venerealis utilizando inmunofluorescencia directa; se lo aisló e identificó mediante PCR y secuenciación del 16S rDNA en el líquido abomasal y en el pulmón. El examen histológico de otros dos fetos reveló encefalitis necrotizante no supurativa, miositis linfohistiocítica y miocarditis, y nefritis linfocítica intersticial. En estos fetos se detectó intralesionalmente el antígeno de N. caninum mediante análisis inmunohistoquímico, y se amplificó el ADN de N. caninum mediante PCR en tejido cerebral fijado con formalina y embebido en parafina. Se detectaron anticuerpos contra N. caninum mediante inmunofluorescencia indirecta en 10 de 27 vacas, con títulos de entre 1/200 y 1/3200. Los resultados indican que dos microorganismos abortígenos pueden coexistir y provocar abortos contemporáneos en un hato. Subrayamos la importancia de realizar pruebas diagnósticas múltiples en diversas madres abortadas y fetos del mismo hato para obtener una confirmación etiológica del síndrome de aborto bovino.
653 $aBovine abortion
653 $aCampylobacter fetus subsp. venerealis
653 $aDiagnosis of abortion
653 $aNeospora caninum
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aCAFFARENA, D.
700 1 $aFRAGA, M.
700 1 $aSILVEIRA, C.S.
700 1 $aGIANNITTI, F.
700 1 $aCANTÓN, G.
700 1 $aHECKER, Y.P.
700 1 $aSUANES, A.
700 1 $aRIET-CORREA, F.
773 $tRevista Mexicana De Ciencias Pecuarias, 2019, Volume 10, Issue 4, 2019, Pages 1054-1063. OPEN ACCESS. Doi: 10.22319/rmcp.v10i4.5008
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